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Proteintech primary antibodies include rabbit anti dnajc7
$A CRISPRi screen of molecular chaperones identifies <t>DNAJC7</t> as a modifier of polyQ aggregation. A , workflow of CRISPRi screen to identify molecular chaperone modifiers of polyQ aggregation in NLS-FRET-Q79 cells. Created with BioRender. B , volcano plot CRISPRi screen results, highlighting DNAJ protein and proteasomal subunit hits. C , flow cytometry plots of NLS-FRET-Q79 cells at 4 days of doxycycline with or without proteasomal inhibitor (carfilzomib). D and E , Western blot ( D ) and quantification ( E ) of DNAJC7 levels in NLS-FRET-Q79 cells expressing nontargeting control (NTC) sgRNAs or sgRNAs targeting DNAJC7. Each lane represents lysate from an independent well; bars and error bars indicate mean ± SD, and points represent n = 3 wells. Signal intensities were normalized to total protein and then to sgNTC#1. F , results of flow cytometry measuring fold change in the fraction of FRET-high cells after 5 days of doxycycline in sgRNA + cells transduced with sgNTC or sgDNAJC7. Data are presented as fold change relative to the mean of the sgNTCs for each independent experiment. Bars and error bars represent mean ± SD, and points represent n = 3 independent experiments. ∗∗ p < 0.05, one-sample t test compared with sgNTCs (fold change = 1.0). CRISPRi, CRISPR interference. NLS, nuclear localization signal; polyQ, polyglutamine; sgRNA, single guide RNA.$
Primary Antibodies Include Rabbit Anti Dnajc7, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/11090+1+ap/pmc12993198-301-0-5?v=Proteintech
Average 93 stars, based on 13 article reviews

primary antibodies include rabbit anti dnajc7 - by Bioz Stars, 2026-07

93/100 stars

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1) Product Images from "The Hsp40 cochaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation"

Article Title: The Hsp40 cochaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation

Journal: The Journal of Biological Chemistry

doi: 10.1016/j.jbc.2026.111292

$A CRISPRi screen of molecular chaperones identifies DNAJC7 as a modifier of polyQ aggregation. A , workflow of CRISPRi screen to identify molecular chaperone modifiers of polyQ aggregation in NLS-FRET-Q79 cells. Created with BioRender. B , volcano plot CRISPRi screen results, highlighting DNAJ protein and proteasomal subunit hits. C , flow cytometry plots of NLS-FRET-Q79 cells at 4 days of doxycycline with or without proteasomal inhibitor (carfilzomib). D and E , Western blot ( D ) and quantification ( E ) of DNAJC7 levels in NLS-FRET-Q79 cells expressing nontargeting control (NTC) sgRNAs or sgRNAs targeting DNAJC7. Each lane represents lysate from an independent well; bars and error bars indicate mean ± SD, and points represent n = 3 wells. Signal intensities were normalized to total protein and then to sgNTC#1. F , results of flow cytometry measuring fold change in the fraction of FRET-high cells after 5 days of doxycycline in sgRNA + cells transduced with sgNTC or sgDNAJC7. Data are presented as fold change relative to the mean of the sgNTCs for each independent experiment. Bars and error bars represent mean ± SD, and points represent n = 3 independent experiments. ∗∗ p < 0.05, one-sample t test compared with sgNTCs (fold change = 1.0). CRISPRi, CRISPR interference. NLS, nuclear localization signal; polyQ, polyglutamine; sgRNA, single guide RNA.$
Figure Legend Snippet: A CRISPRi screen of molecular chaperones identifies DNAJC7 as a modifier of polyQ aggregation. A , workflow of CRISPRi screen to identify molecular chaperone modifiers of polyQ aggregation in NLS-FRET-Q79 cells. Created with BioRender. B , volcano plot CRISPRi screen results, highlighting DNAJ protein and proteasomal subunit hits. C , flow cytometry plots of NLS-FRET-Q79 cells at 4 days of doxycycline with or without proteasomal inhibitor (carfilzomib). D and E , Western blot ( D ) and quantification ( E ) of DNAJC7 levels in NLS-FRET-Q79 cells expressing nontargeting control (NTC) sgRNAs or sgRNAs targeting DNAJC7. Each lane represents lysate from an independent well; bars and error bars indicate mean ± SD, and points represent n = 3 wells. Signal intensities were normalized to total protein and then to sgNTC#1. F , results of flow cytometry measuring fold change in the fraction of FRET-high cells after 5 days of doxycycline in sgRNA + cells transduced with sgNTC or sgDNAJC7. Data are presented as fold change relative to the mean of the sgNTCs for each independent experiment. Bars and error bars represent mean ± SD, and points represent n = 3 independent experiments. ∗∗ p < 0.05, one-sample t test compared with sgNTCs (fold change = 1.0). CRISPRi, CRISPR interference. NLS, nuclear localization signal; polyQ, polyglutamine; sgRNA, single guide RNA.

Techniques Used: Flow Cytometry, Western Blot, Expressing, Control, Transduction, CRISPR

$DNAJC7 suppresses mutant Huntingtin exon 1 (HTTex1) aggregation and colocalizes with inclusions. A , schematic of a lentiviral construct encoding a doxycycline-inducible eGFP-tagged fragment of HTTex1 containing 72 glutamines (GFP-HTTex1-Q72) used to generate a monoclonal HEK293T CRISPRi cell line. B , fluorescence micrograph of GFP-HTTex1-Q72 cells at 7 days of doxycycline treatment before and after treatment with Triton X-100. C , results of flow cytometry experiments measuring the fraction of total events containing detergent-resistant GFP + cells comparing those stably transduced with NTC or DNAJC7-targeting sgRNAs and normalized to the mean of NTCs. Data represent mean ± SD from n = 4 independent experiments. ∗∗ p < 0.05, one-sample t test compared with sgNTCs (fold change = 1.0). D , fluorescence micrographs of HEK293T cells 48 h after transient cotransfection of GFP-HTTex1-Q72 and either mTagBFP2 (BFP) alone or BFP-tagged DNAJC7. Arrows indicate cytoplasmic aggregates. E , flow cytometry plotting GFP-height versus GFP-width (pulse shape analysis) of HEK293T cells transfected with GFP-HTTex1-Q72 for 48 h. The detergent-resistant population of cells is designated as aggregate positive (Agg + ). F , results of flow cytometry experiments measuring Agg + cells in HEK293T cells 48 h after transient cotransfection with GFP-HTTex1-Q72 and either BFP or BFP-DNAJC7. Bars and error bars represent mean ± SD from n = 4 independent biological replicates (indicated by color), with each performed in technical triplicate. ∗ p < 0.05 by Student’s t test using mean values of technical replicates. The scale bars represent 10 μm. CRISPRi, CRISPR interference; eGFP, enhanced GFP; HEK293T, human embryonic kidney 293T cell line; NTC, nontargeting control; sgRNA, single guide RNA.$
Figure Legend Snippet: DNAJC7 suppresses mutant Huntingtin exon 1 (HTTex1) aggregation and colocalizes with inclusions. A , schematic of a lentiviral construct encoding a doxycycline-inducible eGFP-tagged fragment of HTTex1 containing 72 glutamines (GFP-HTTex1-Q72) used to generate a monoclonal HEK293T CRISPRi cell line. B , fluorescence micrograph of GFP-HTTex1-Q72 cells at 7 days of doxycycline treatment before and after treatment with Triton X-100. C , results of flow cytometry experiments measuring the fraction of total events containing detergent-resistant GFP + cells comparing those stably transduced with NTC or DNAJC7-targeting sgRNAs and normalized to the mean of NTCs. Data represent mean ± SD from n = 4 independent experiments. ∗∗ p < 0.05, one-sample t test compared with sgNTCs (fold change = 1.0). D , fluorescence micrographs of HEK293T cells 48 h after transient cotransfection of GFP-HTTex1-Q72 and either mTagBFP2 (BFP) alone or BFP-tagged DNAJC7. Arrows indicate cytoplasmic aggregates. E , flow cytometry plotting GFP-height versus GFP-width (pulse shape analysis) of HEK293T cells transfected with GFP-HTTex1-Q72 for 48 h. The detergent-resistant population of cells is designated as aggregate positive (Agg + ). F , results of flow cytometry experiments measuring Agg + cells in HEK293T cells 48 h after transient cotransfection with GFP-HTTex1-Q72 and either BFP or BFP-DNAJC7. Bars and error bars represent mean ± SD from n = 4 independent biological replicates (indicated by color), with each performed in technical triplicate. ∗ p < 0.05 by Student’s t test using mean values of technical replicates. The scale bars represent 10 μm. CRISPRi, CRISPR interference; eGFP, enhanced GFP; HEK293T, human embryonic kidney 293T cell line; NTC, nontargeting control; sgRNA, single guide RNA.

Techniques Used: Mutagenesis, Construct, Fluorescence, Flow Cytometry, Stable Transfection, Transduction, Cotransfection, Transfection, CRISPR, Control

$Chaperone CRISPRi screening reveals limited suppression of polyG aggregation despite effects by DNAJC7 overexpression. A , volcano plot showing results of a CRISPRi screen for molecular chaperone modifiers of polyG aggregation in the NLS-FRET-G100 cell line. B , pairwise comparison of gene scores between the polyG screen (from A ) and the polyQ screen ( B ). C , results of flow cytometry experiments measuring fold change in the fraction of FRET-high cells after 5 days of doxycycline in sgRNA + cells transduced with NTC or DNAJC7. Data represent mean ± SD from n = 3 independent experiments. D , representative fluorescence micrographs of HEK293T cells transiently cotransfected with NLS-mScarlet- and NLS-mNeonGreen-tagged uN2C-G100 together with either BFP or BFP-DNAJC7; only the mNeonGreen channel is shown. The scale bar represents 10 μm. E , flow cytometry quantification of the percentage of FRET-high cells relative to total cells expressing both mNeonGreen and mScarlet. Bars indicate mean ± SD from n = 3 independent experiments. ∗ p < 0.05 by Student’s t test. CRISPRi, CRISPR interference; HEK293T, human embryonic kidney 293T cell line; NLS, nuclear localization signal; NTC, nontargeting control; polyG, polyglucine; polyQ, polyglutamine; sgRNA, single guide RNA.$
Figure Legend Snippet: Chaperone CRISPRi screening reveals limited suppression of polyG aggregation despite effects by DNAJC7 overexpression. A , volcano plot showing results of a CRISPRi screen for molecular chaperone modifiers of polyG aggregation in the NLS-FRET-G100 cell line. B , pairwise comparison of gene scores between the polyG screen (from A ) and the polyQ screen ( B ). C , results of flow cytometry experiments measuring fold change in the fraction of FRET-high cells after 5 days of doxycycline in sgRNA + cells transduced with NTC or DNAJC7. Data represent mean ± SD from n = 3 independent experiments. D , representative fluorescence micrographs of HEK293T cells transiently cotransfected with NLS-mScarlet- and NLS-mNeonGreen-tagged uN2C-G100 together with either BFP or BFP-DNAJC7; only the mNeonGreen channel is shown. The scale bar represents 10 μm. E , flow cytometry quantification of the percentage of FRET-high cells relative to total cells expressing both mNeonGreen and mScarlet. Bars indicate mean ± SD from n = 3 independent experiments. ∗ p < 0.05 by Student’s t test. CRISPRi, CRISPR interference; HEK293T, human embryonic kidney 293T cell line; NLS, nuclear localization signal; NTC, nontargeting control; polyG, polyglucine; polyQ, polyglutamine; sgRNA, single guide RNA.

Techniques Used: Over Expression, Comparison, Flow Cytometry, Transduction, Fluorescence, Expressing, CRISPR, Control

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Journal: The Journal of Biological Chemistry

Article Title: The Hsp40 cochaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation

doi: 10.1016/j.jbc.2026.111292

Figure Lengend Snippet: A CRISPRi screen of molecular chaperones identifies DNAJC7 as a modifier of polyQ aggregation. A , workflow of CRISPRi screen to identify molecular chaperone modifiers of polyQ aggregation in NLS-FRET-Q79 cells. Created with BioRender. B , volcano plot CRISPRi screen results, highlighting DNAJ protein and proteasomal subunit hits. C , flow cytometry plots of NLS-FRET-Q79 cells at 4 days of doxycycline with or without proteasomal inhibitor (carfilzomib). D and E , Western blot ( D ) and quantification ( E ) of DNAJC7 levels in NLS-FRET-Q79 cells expressing nontargeting control (NTC) sgRNAs or sgRNAs targeting DNAJC7. Each lane represents lysate from an independent well; bars and error bars indicate mean ± SD, and points represent n = 3 wells. Signal intensities were normalized to total protein and then to sgNTC#1. F , results of flow cytometry measuring fold change in the fraction of FRET-high cells after 5 days of doxycycline in sgRNA + cells transduced with sgNTC or sgDNAJC7. Data are presented as fold change relative to the mean of the sgNTCs for each independent experiment. Bars and error bars represent mean ± SD, and points represent n = 3 independent experiments. ∗∗ p < 0.05, one-sample t test compared with sgNTCs (fold change = 1.0). CRISPRi, CRISPR interference. NLS, nuclear localization signal; polyQ, polyglutamine; sgRNA, single guide RNA.

Article Snippet: Primary antibodies include rabbit anti-DNAJC7 (Proteintech, 11090-1-AP, 1:2000 dilution) and mouse anti-GAPDH (Proteintech, 60004-1-Ig; 1:2000 dilution).

Techniques: Flow Cytometry, Western Blot, Expressing, Control, Transduction, CRISPR

Journal: The Journal of Biological Chemistry

Article Title: The Hsp40 cochaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation

doi: 10.1016/j.jbc.2026.111292

Figure Lengend Snippet: DNAJC7 suppresses mutant Huntingtin exon 1 (HTTex1) aggregation and colocalizes with inclusions. A , schematic of a lentiviral construct encoding a doxycycline-inducible eGFP-tagged fragment of HTTex1 containing 72 glutamines (GFP-HTTex1-Q72) used to generate a monoclonal HEK293T CRISPRi cell line. B , fluorescence micrograph of GFP-HTTex1-Q72 cells at 7 days of doxycycline treatment before and after treatment with Triton X-100. C , results of flow cytometry experiments measuring the fraction of total events containing detergent-resistant GFP + cells comparing those stably transduced with NTC or DNAJC7-targeting sgRNAs and normalized to the mean of NTCs. Data represent mean ± SD from n = 4 independent experiments. ∗∗ p < 0.05, one-sample t test compared with sgNTCs (fold change = 1.0). D , fluorescence micrographs of HEK293T cells 48 h after transient cotransfection of GFP-HTTex1-Q72 and either mTagBFP2 (BFP) alone or BFP-tagged DNAJC7. Arrows indicate cytoplasmic aggregates. E , flow cytometry plotting GFP-height versus GFP-width (pulse shape analysis) of HEK293T cells transfected with GFP-HTTex1-Q72 for 48 h. The detergent-resistant population of cells is designated as aggregate positive (Agg + ). F , results of flow cytometry experiments measuring Agg + cells in HEK293T cells 48 h after transient cotransfection with GFP-HTTex1-Q72 and either BFP or BFP-DNAJC7. Bars and error bars represent mean ± SD from n = 4 independent biological replicates (indicated by color), with each performed in technical triplicate. ∗ p < 0.05 by Student’s t test using mean values of technical replicates. The scale bars represent 10 μm. CRISPRi, CRISPR interference; eGFP, enhanced GFP; HEK293T, human embryonic kidney 293T cell line; NTC, nontargeting control; sgRNA, single guide RNA.

Article Snippet: Primary antibodies include rabbit anti-DNAJC7 (Proteintech, 11090-1-AP, 1:2000 dilution) and mouse anti-GAPDH (Proteintech, 60004-1-Ig; 1:2000 dilution).

Techniques: Mutagenesis, Construct, Fluorescence, Flow Cytometry, Stable Transfection, Transduction, Cotransfection, Transfection, CRISPR, Control

Journal: The Journal of Biological Chemistry

Article Title: The Hsp40 cochaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregation

doi: 10.1016/j.jbc.2026.111292

Figure Lengend Snippet: Chaperone CRISPRi screening reveals limited suppression of polyG aggregation despite effects by DNAJC7 overexpression. A , volcano plot showing results of a CRISPRi screen for molecular chaperone modifiers of polyG aggregation in the NLS-FRET-G100 cell line. B , pairwise comparison of gene scores between the polyG screen (from A ) and the polyQ screen ( B ). C , results of flow cytometry experiments measuring fold change in the fraction of FRET-high cells after 5 days of doxycycline in sgRNA + cells transduced with NTC or DNAJC7. Data represent mean ± SD from n = 3 independent experiments. D , representative fluorescence micrographs of HEK293T cells transiently cotransfected with NLS-mScarlet- and NLS-mNeonGreen-tagged uN2C-G100 together with either BFP or BFP-DNAJC7; only the mNeonGreen channel is shown. The scale bar represents 10 μm. E , flow cytometry quantification of the percentage of FRET-high cells relative to total cells expressing both mNeonGreen and mScarlet. Bars indicate mean ± SD from n = 3 independent experiments. ∗ p < 0.05 by Student’s t test. CRISPRi, CRISPR interference; HEK293T, human embryonic kidney 293T cell line; NLS, nuclear localization signal; NTC, nontargeting control; polyG, polyglucine; polyQ, polyglutamine; sgRNA, single guide RNA.

Article Snippet: Primary antibodies include rabbit anti-DNAJC7 (Proteintech, 11090-1-AP, 1:2000 dilution) and mouse anti-GAPDH (Proteintech, 60004-1-Ig; 1:2000 dilution).

Techniques: Over Expression, Comparison, Flow Cytometry, Transduction, Fluorescence, Expressing, CRISPR, Control

Representative confocal micrographs of AD293 cells treated with 0.5 M sorbitol for 1 h. Cells transiently transfected with WDR62-mCherry and either the HSP co-chaperones ( A ) BAG2-EGFP, ( B ) EGFP-DNAJC7, ( C ) EGFP-STIP1, or de novo purine biosynthesis (DNPB) enzymes ( D ) PFAS-EGFP, ( E ) PPAT-EGFP, ( F ) GART-EGFP or ( G ) PAICS-EGFP. Co-localisation of each signal is indicated by fluorescence intensity plots to the right of each set of images ( y -axis represents fluorescence intensity (a.u.), x -axis (µm) represents the length of the white line drawn on the ROI). ( H ) Bar graph representing the co-localisation between WDR62-mCherry and EGFP signal for each respective protein (mean ± SD). Each dot represents the Person’s correlation coefficient for a single ROI. ( I ) Confocal micrographs of AD293 cells co-transfected with WDR62-mCherry and PFAS-EGFP, in either control (top) or purine-depleted (bottom) conditions. Bar graph on the right represents co-localisation between WDR62-mCherry and PFAS-EGFP (mean ± SD) ( ***P = 0.0004). ( J ) Co-immunoprecipitation and immunoblot of myc-PFAS and HA-WDR62. ( K ) The association of endogenous WDR62 with BAG2 and PFAS as measured by PLA (grey spots) with DAPI-stained nuclei in blue. PLA signal is observed under basal (control) and sorbitol-treated (0.5 M, 1 h) conditions. ( L ) Quantification of the number of PLA puncta per cell for WDR62/BAG2 and WDR62/PFAS. Data represent n = 3 biological replicates. P values calculated based on mean values using a one-way ANOVA for ( H ) and a two-tailed unpaired T test for ( I , L ) (* P < 0.05, ** P < 0.005, *** P < 0.001, **** P < 0.0001, n.s. is P > 0.05). All scale bars represent 20 µm. .

Journal: The EMBO Journal

Article Title: Microcephaly-associated protein WDR62 supports purine metabolism by interacting with co-chaperone BAG2

doi: 10.1038/s44318-026-00724-0

Figure Lengend Snippet: Representative confocal micrographs of AD293 cells treated with 0.5 M sorbitol for 1 h. Cells transiently transfected with WDR62-mCherry and either the HSP co-chaperones ( A ) BAG2-EGFP, ( B ) EGFP-DNAJC7, ( C ) EGFP-STIP1, or de novo purine biosynthesis (DNPB) enzymes ( D ) PFAS-EGFP, ( E ) PPAT-EGFP, ( F ) GART-EGFP or ( G ) PAICS-EGFP. Co-localisation of each signal is indicated by fluorescence intensity plots to the right of each set of images ( y -axis represents fluorescence intensity (a.u.), x -axis (µm) represents the length of the white line drawn on the ROI). ( H ) Bar graph representing the co-localisation between WDR62-mCherry and EGFP signal for each respective protein (mean ± SD). Each dot represents the Person’s correlation coefficient for a single ROI. ( I ) Confocal micrographs of AD293 cells co-transfected with WDR62-mCherry and PFAS-EGFP, in either control (top) or purine-depleted (bottom) conditions. Bar graph on the right represents co-localisation between WDR62-mCherry and PFAS-EGFP (mean ± SD) ( ***P = 0.0004). ( J ) Co-immunoprecipitation and immunoblot of myc-PFAS and HA-WDR62. ( K ) The association of endogenous WDR62 with BAG2 and PFAS as measured by PLA (grey spots) with DAPI-stained nuclei in blue. PLA signal is observed under basal (control) and sorbitol-treated (0.5 M, 1 h) conditions. ( L ) Quantification of the number of PLA puncta per cell for WDR62/BAG2 and WDR62/PFAS. Data represent n = 3 biological replicates. P values calculated based on mean values using a one-way ANOVA for ( H ) and a two-tailed unpaired T test for ( I , L ) (* P < 0.05, ** P < 0.005, *** P < 0.001, **** P < 0.0001, n.s. is P > 0.05). All scale bars represent 20 µm. .

Article Snippet: DNAJC7 (rabbit polyclonal) , Proteintech , 11090-1-AP.

Techniques: Transfection, Fluorescence, Control, Immunoprecipitation, Western Blot, Staining, Two Tailed Test

Figure 2. DnaJC7 knockout (KO) uniquely extends tau seed lifespan in dividing cells. (A) Schematic showing the HEK293 OFF1::DS10 system. A selection of the hits from the proteomics screen was knocked out in these cells. The cells are then allowed to grow with tau expression turned OFF for 0–5days before resuming tau expression. Error bars represent the SEM of six technical replicates. (B) The persistence of tau aggregates in OFF1::DS10 cells with the indicated KO was quantified following 3 (orange bars) or 5 (purple bars) days of repressed tau expression. Error bars represent the SEM of six technical replicates. (C) Confocal microscopy images showing tau aggregate organization in the DnaJC7 KO and nontargeting control cells following 0 or 5days of repression of tau expression. Scale bars denote 20 µm. (D) Extended time course for tau aggregate clearance in the OFF1::DS10 system with DnaJC7 KO (orange) and the nontargeting (purple) and untreated (green) controls. Error bars represent SEM of six technical replicates. *=p < 0.05, **=p < 0.01, ***=p < 0.001, ****=p < 0.0001.

Journal: eLife

Article Title: DnaJC7 specifically regulates tau seeding

doi: 10.7554/elife.86936

Figure Lengend Snippet: Figure 2. DnaJC7 knockout (KO) uniquely extends tau seed lifespan in dividing cells. (A) Schematic showing the HEK293 OFF1::DS10 system. A selection of the hits from the proteomics screen was knocked out in these cells. The cells are then allowed to grow with tau expression turned OFF for 0–5days before resuming tau expression. Error bars represent the SEM of six technical replicates. (B) The persistence of tau aggregates in OFF1::DS10 cells with the indicated KO was quantified following 3 (orange bars) or 5 (purple bars) days of repressed tau expression. Error bars represent the SEM of six technical replicates. (C) Confocal microscopy images showing tau aggregate organization in the DnaJC7 KO and nontargeting control cells following 0 or 5days of repression of tau expression. Scale bars denote 20 µm. (D) Extended time course for tau aggregate clearance in the OFF1::DS10 system with DnaJC7 KO (orange) and the nontargeting (purple) and untreated (green) controls. Error bars represent SEM of six technical replicates. *=p < 0.05, **=p < 0.01, ***=p < 0.001, ****=p < 0.0001.

Article Snippet: The following antibodies were used for immunoblotting: rabbit polyclonal anti- DnaJC7 (Proteintech, 11090- 1- AP) at a 1:2000 dilution; rabbit polyclonal anti- DnaJB6 (Proteintech, 11707- 1- AP) at a 1:2000 dilution; rabbit polyclonal anti- Beta- Tubulin (Proteintech, 10094- 1- AP) at a 1:5000 dilution; a secondary donkey- anti- rabbit HRP- linked F(ab’)2 (Cytiva, NA9340- 1ML) at a 1:5000 dilution when blotting for DnaJC7, a 1:4000 dilution when blotting for DnaJB6, and a 1:5000 dilution when blotting for Tubulin; mouse monoclonal anti- Beta- Actin (Proteintech, 66009- 1- Ig) at a 1:5000 dilution; mouse monoclonal anti- GAPDH (Proteintech, 60004- 1- Ig) at a 1:10,000 dilution; and a secondary goat- antimouse H&L (HRP) (Abcam, ab6789) at a 1:5000 dilution when blotting for Beta- Actin and 1:10,000 when blotting for Beta- Tubulin.

Techniques: Knock-Out, Selection, Expressing, Confocal Microscopy, Control

Journal: eLife

Article Title: DnaJC7 specifically regulates tau seeding

doi: 10.7554/eLife.86936

Figure Lengend Snippet:

Article Snippet: Antibody , Rabbit polyclonal anti-DnaJC7 , Proteintech , 11090-1-AP , 1:2000 dilution.

Techniques: Produced, Stable Transfection, Recombinant, Sequencing, Clone Assay, Construct

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